Fixation of human embryological and cytological material


Fixation of human embryological and cytological material

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ABSTRACT IT is known that it is very difficult to obtain well-preserved human material. Few medical men realise that five or ten minutes after the tissue has been removed, or after death,


plasmolytic changes supervene, and in the fixed and stained sections the chromosomes have clumped badly, and the delicate lipoid cytoplasmic organellæ have become abnormal, or completely


macerated. Recently, I have been studying certain human material, and find that nearly every type of histological preparation can be made from two fixing fluids as follows: one of the


surgeon's assistants is given two bottles, one of Da Fano's cobalt nitrate formalin fluid, and one of Regaud's formalin-bichromate fluid. Pieces of tissue as large as the


thumb may be thrown into these bottles, and afterwards cut into smaller pieces when they have been brought to the laboratory. It is better to change into new fluid at once, especially if the


organ is very vascular, and the fixing fluid mixed with blood. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS


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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS PARAFFIN-EMBEDDING FOR LARGE VOLUME BIO-TISSUE Article Open access 28 July 2020


HFRUIT: AN OPTIMIZED AGENT FOR OPTICAL CLEARING OF DII-STAINED ADULT HUMAN BRAIN TISSUE Article Open access 19 June 2020 ADVANCED TECHNOLOGIES FOR THE PRESERVATION OF MAMMALIAN BIOSPECIMENS


Article 23 August 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Zoological Department, Dublin University, J. BRONTË GATENBY Authors * J. BRONTË GATENBY View author publications You can


also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE GATENBY, J. Fixation of Human Embryological and


Cytological Material. _Nature_ 112, 830 (1923). https://doi.org/10.1038/112830a0 Download citation * Issue Date: 08 December 1923 * DOI: https://doi.org/10.1038/112830a0 SHARE THIS ARTICLE


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