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ABSTRACT The retinoblastoma tumor suppressor protein and its family members, p107 and p130, are major regulators of the mammalian cell cycle. They exert their growth suppressive effects at

least in part by binding the E2F family of transcription factors and inhibiting their transcriptional activity. Agents that disrupt the interaction between Rb family proteins and E2F promote

cell proliferation. Here we describe the characterization of a novel interaction between Rb family proteins and a potential tumor suppressor protein, prohibitin. Prohibitin physically

interacts with all three Rb family proteins _IN VITRO_ and _IN VIVO_, and was very effective in repressing E2F-mediated transcription. Prohibitin could inhibit the activity of E2Fs 1, 2, 3,

4 and 5, but could not affect the activity of promoters lacking an E2F site. Surprisingly, prohibitin-mediated repression of E2F could not be reversed by adenovirus E1A protein. A prohibitin

mutant that could not bind to Rb was impaired in its ability to repress E2F activity and inhibit cell proliferation. We believe that prohibitin is a novel regulator of E2F activity that

responds to specific signaling cascades. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS CELL CYCLE REGULATION: P53-P21-RB SIGNALING Article Open access 31 March 2022 AN INTERMEDIATE RB–E2F ACTIVITY

STATE SAFEGUARDS PROLIFERATION COMMITMENT Article Open access 26 June 2024 HDAC ACTIVITY IS DISPENSABLE FOR REPRESSION OF CELL-CYCLE GENES BY DREAM AND E2F:RB COMPLEXES Article Open access

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cDNA and antibodies, as well as for helpful suggestions. This study was supported by a grant from the NCI (CA63136). SPC is a recipient of the Irma-Hirschl Trust Research Award. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Department of Pathology, College of Physicians and Surgeons, Columbia University, 630W 168th Street, New York, 10032, NY, USA Sheng Wang, Niharika Nath

 & Srikumar Chellappan * Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, 630W 168th Street, New York, 10032, NY, USA Matthew Adlam

Authors * Sheng Wang View author publications You can also search for this author inPubMed Google Scholar * Niharika Nath View author publications You can also search for this author

inPubMed Google Scholar * Matthew Adlam View author publications You can also search for this author inPubMed Google Scholar * Srikumar Chellappan View author publications You can also

search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Wang, S., Nath, N., Adlam, M. _et al._ Prohibitin, a

potential tumor suppressor, interacts with RB and regulates E2F function. _Oncogene_ 18, 3501–3510 (1999). https://doi.org/10.1038/sj.onc.1202684 Download citation * Received: 01 September

1998 * Revised: 20 November 1998 * Accepted: 13 January 1999 * Published: 15 June 1999 * Issue Date: 10 June 1999 * DOI: https://doi.org/10.1038/sj.onc.1202684 SHARE THIS ARTICLE Anyone you

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Springer Nature SharedIt content-sharing initiative KEYWORDS * Rb * E2F * cell cycle * transformation * tumor suppression