Leukaemogenic potency of WEHI-3B cells grown in vitro or in leukaemic mice
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In order to quantify contaminating leukaemia inducing cells in blood or bone marrow from WEHI-3B bearing BALB/c mice (injected with 105 WEHI-3B suspension culture cells), in vitro colony
forming leukaemia cells (CFU-L) and survival rate and/or survival time of mice transplanted with cells from WEHI bearing mice or suspension culture were correlated. ED50 (inoculum inducing
leukaemia in 50% of the animals) was 109 CFU-L (33–361) from suspension culture cells. Three weeks after initiation of leukaemia 2 × 105 BM or 0.5–2.0 × 106 blood cells induced 100%
mortality of recipients. After mobilisation with CY or CY and G-CSF, the same amount of blood or BM cells did not induce leukaemia in recipients. A significant negative correlation was found
between the survival time of leukaemic mice and the log number of CFU-L inoculated from in vivosources. In terms of CFU-L cells, leukaemia induction to BM or WBC obtained 3 weeks after
leukaemia induction were more potent; those from BM or WBC also obtained at 3 weeks but after mobilisation were less potent inducers than those from suspension culture. These data suggest
that CFU-L and leukaemogenic cells are associated, but not identical. Bone Marrow Transplantation (2001) 28, 699–704.
We wish to express our thanks to Ms M Renner and Ms O Ullrich for their skilful technical assistance. This work was partly supported by grants 094/2000 and 508/2000 (Hungarian Ministry of
Health) and T 026441 (Hungarian Academy of Sciences).
National Institute of Haematology and Immunology, Budapest, Hungary
Dept of Pharmacology Medical and Health Science Center, University of Debrecen, Hungary
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