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ABSTRACT IN a recent report, Rose1 described the appearance in tissue cultures of crystals which were ‘helical, tubular, ribbon-like, hexagonal, rhomboidal and filamentous’ in shape. He was

unable to identify the nature of the crystals by cytochemical techniques. We were struck by this report because, over a period of years, we also had observed crystals with an identical

variation in morphology in the peritoneal fluid in diffusion chambers implanted intraperitoneally in mice (technique described in ref. 2). Peritoneal fluid enters the chamber by passing

through a Schleieher and Schuell ‘Very Dense’ cellulose membrane filter which has pores of a maximum diameter of 0.1µ through which cells cannot pass. Access through your institution Buy or

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MEMBRANE THICKNESS, LIPID PHASE AND STEROL TYPE ARE DETERMINING FACTORS IN THE PERMEABILITY OF MEMBRANES TO SMALL SOLUTES Article Open access 25 March 2022 CHOLESTEROL ESTERS FORM

SUPERCOOLED LIPID DROPLETS WHOSE NUCLEATION IS FACILITATED BY TRIACYLGLYCEROLS Article Open access 17 February 2023 PHYSICAL BASIS FOR THE DETERMINATION OF LUMEN SHAPE IN A SIMPLE EPITHELIUM

Article Open access 23 September 2021 REFERENCES * Rose, G. G., _Cancer Res._, 23, 279 (1963). CAS  PubMed  Google Scholar  * Shelton, E., and Rice, M. E., _J. Nat. Cancer Inst._, 21, 137

(1958). CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * EMMA SHELTON Present address: Institut de Recherches Scientifiques sur le Cancer, Villejuif (Seine), France

AUTHORS AND AFFILIATIONS * Laboratory of Biochemistry, National Cancer Institute, EMMA SHELTON, THEODORE T. OTANI & HENRY M. FALES * Laboratory of Metabolism, National Heart Institute,

National Institutes of Health, Bethesda, 14, Maryland EMMA SHELTON * Institut de Recherches Scientifiques sur le Cancer, Villejuif (Seine), France THEODORE T. OTANI & HENRY M. FALES

Authors * EMMA SHELTON View author publications You can also search for this author inPubMed Google Scholar * THEODORE T. OTANI View author publications You can also search for this author

inPubMed Google Scholar * HENRY M. FALES View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS

ARTICLE CITE THIS ARTICLE SHELTON, E., OTANI, T. & FALES, H. Accumulation of Cholesterol Crystals in Diffusion Chambers implanted in Mice. _Nature_ 202, 1229–1230 (1964).

https://doi.org/10.1038/2021229a0 Download citation * Issue Date: 20 June 1964 * DOI: https://doi.org/10.1038/2021229a0 SHARE THIS ARTICLE Anyone you share the following link with will be

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