RNA retrieved from intact tissue
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A technique can snatch RNA out of a single cell in live, intact tissue, revealing the genes being expressed by that cell.
Other methods of single-cell RNA retrieval tend to disrupt the surrounding tissue before the RNA is captured. James Eberwine of the University of Pennsylvania in Philadelphia and his
colleagues created a molecule called a TIVA tag that penetrates cells.
When hit with a beam of light, the tag binds to RNA that is being made from its DNA template. By adding the tag to human and mouse brain tissue and shining a laser on individual cells, the
researchers could activate the tag, retrieve the RNA and sequence it.
The approach could reveal how a cell's natural microenvironment affects its activity.
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