Extraction and preliminary characterization of a human bronchogenic carcinoma antigen
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ABSTRACT Saline extracts of human bronchogenic tumours, soluble in 50% saturated ammonium sulphate and also fractions from Sephadex G-200 chromatography were used to raise antisera in
rabbits. After absorbing the antisera with normal tissue extracts, direct Ouchterlony tests were performed against tumour (adenocarcinomata and squamous cell carcinomata) and normal
extracts. A precipitin reaction was given with all 11 tumour extracts tested at a concentration of 5 mg/ml whereas all the 9 normal lung control extracts did not react at concentrations up
to 100 mg/ml. The possibility that this reaction could be related to histocompatibility differences between individuals is ruled out by the fact that in two cases tumour and normal tissue
were obtained from the same patient. These studies and also precipitin-inhibition experiments have confirmed the existence of antigen associated with bronchial carcinomata and have shown
that, although the antigen or a cross-reacting antigen is present in normal lung tissue, the amounts are small in comparison with the amounts extracted from tumour. Antigenic activity was
contained in a single absorbance peak when fractionated by Sephadex G-200 chromatography and its elution volume indicated a molecular weight of approximately 4-0 times 10(4)D. Further
purification was achieved using isotachophoresis. Preliminary characterization of the antigen has shown it to be stable at pH 4-5, resistant to heating at 50 degrees C for 30 min, to migrate
on immunoelectrophoresis with a cationic mobility at PH 8-5 and to be immunologically distinct from carcinoembryonic antigen. Access through your institution Buy or subscribe This is a
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G PROTEIN-COUPLED RECEPTOR GPR19 IN NORMAL AND NEOPLASTIC HUMAN TISSUES Article Open access 03 November 2023 THE SIGNIFICANCE OF ELEVATED TUMOR MARKERS AMONG PATIENTS WITH INTERSTITIAL LUNG
DISEASES Article Open access 06 October 2022 IMMUNODOMINANT PROTEINS P1 AND P40/P90 FROM HUMAN PATHOGEN _MYCOPLASMA PNEUMONIAE_ Article Open access 14 October 2020 Authors * M J Frost View
author publications You can also search for this author inPubMed Google Scholar * G T Rogers View author publications You can also search for this author inPubMed Google Scholar * K D
Bagshawe View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Frost, M.,
Rogers, G. & Bagshawe, K. Extraction and preliminary characterization of a human bronchogenic carcinoma antigen. _Br J Cancer_ 31, 379–386 (1975). https://doi.org/10.1038/bjc.1975.77
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