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ABSTRACT Controlled-rate freezing and storage in vapour phase nitrogen are used by most transplantation teams for the cryopreservation and storage of peripheral blood haematopoietic stem

cells (PBSC). In this study, we analysed 666 autologous PBSC transplants after uncontrolled freezing and storage of PBSC at −80 °C. Statistical analysis showed that neutrophil recovery was

associated with both the infused CD34+ cell dose (_P_=0.01) and the post transplantation use of growth factors (_P_<0.001) and that platelet recovery was associated with the infused CD34+

cell dose (_P_<0.001) and with the diagnosis (_P_=0.02). We analysed three groups according to the duration of the cryopreservation period (less than 6 months, between 6 and 12 months or

more than 1 year). Haematopoietic recovery was not found to be adversely affected by longer storage at −80 °C. The haematopoietic recoveries of 50 pairs of sequential transplantations from

the same PBSC mobilization were analysed. Despite prolonged cryopreservation, there were no statistically significant differences in neutrophil (_P_=0.09) or platelet (_P_=0.22) recovery in

the second compared with the first transplant. In conclusion, the long-term storage of PBSC at −80 °C after uncontrolled-rate freezing is an easy and comparatively inexpensive

cryopreservation method that leads to successful haematopoietic recovery even after prolonged storage. Access through your institution Buy or subscribe This is a preview of subscription

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references ACKNOWLEDGEMENTS No source of support (grants, equipment, drugs) was required to conduct this study. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratoire d’hématologie,

Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium G Detry, B Husson & L Boon-Falleur * Service de Thérapie cellulaire et Hématologie Clinique, Université d’Auvergne, CHU Estaing,

Clermont-Ferrand, France L Calvet, A Cabrespine, J O Bay & O Tournilhac * Service d’hématologie, Centre Hospitalier de Jolimont, Haine Saint Paul, Belgium N Straetmans, C Ravoet, H

Petre & A Delannoy * Centre régional de cancérologie et thérapie cellulaire pédiatrique, Centre de Biothérapie d’Auvergne, CHU Estaing, Clermont-Ferrand, France C Paillard, E Merlin 

& P Halle Authors * G Detry View author publications You can also search for this author inPubMed Google Scholar * L Calvet View author publications You can also search for this author

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publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to G Detry. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no

conflict of interest. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Detry, G., Calvet, L., Straetmans, N. _et al._ Impact of uncontrolled freezing and

long-term storage of peripheral blood stem cells at −80 °C on haematopoietic recovery after autologous transplantation. Report from two centres. _Bone Marrow Transplant_ 49, 780–785 (2014).

https://doi.org/10.1038/bmt.2014.53 Download citation * Received: 30 July 2013 * Revised: 16 January 2014 * Accepted: 31 January 2014 * Published: 31 March 2014 * Issue Date: June 2014 *

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