Secondary constrictions and NORs of of Lathyrus investigated by silver staining and in-situ hybridization
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A variety of karyotypic differences were seen among six Lathyrus species. In part these differences are the result of different numbers of metacentric and acrocentric chromosomes in the
complement but there were also clear differences in the number(s) and position(s) of secondary constrictions. Silver staining and in-situ hybridization were used to identify the nucleolar
organizer regions (NORs). Silver staining also occurred at the centromere in most species and in addition at a number of non-centromeric sites in L. tingitanus. Some of these additional
sites coincide with allocyclic regions revealed by the use of air-dried preparations. Unusual ‘extended’ centromeres were observed in these air-dried preparations of two of the species.
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