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ABSTRACT A variety of karyotypic differences were seen among six _Lathyrus_ species. In part these differences are the result of different numbers of metacentric and acrocentric chromosomes

in the complement but there were also clear differences in the number(s) and position(s) of secondary constrictions. Silver staining and in-situ hybridization were used to identify the

nucleolar organizer regions (NORs). Silver staining also occurred at the centromere in most species and in addition at a number of non-centromeric sites in _L. tingitanus_. Some of these

additional sites coincide with allocyclic regions revealed by the use of air-dried preparations. Unusual ‘extended’ centromeres were observed in these air-dried preparations of two of the

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Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AB, UK B G Murray & M D Bennett * DSIR Fruit and Trees, Private Bag, Auckland, New Zealand K R W Hammett Authors *

B G Murray View author publications You can also search for this author inPubMed Google Scholar * M D Bennett View author publications You can also search for this author inPubMed Google

Scholar * K R W Hammett View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Murray, B., Bennett, M. & Hammett, K. Secondary constrictions and NORs of of _Lathyrus_ investigated by silver staining and _in-situ_ hybridization. _Heredity_ 68, 473–478

(1992). https://doi.org/10.1038/hdy.1992.68 Download citation * Received: 19 June 1991 * Issue Date: 01 May 1992 * DOI: https://doi.org/10.1038/hdy.1992.68 SHARE THIS ARTICLE Anyone you

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Springer Nature SharedIt content-sharing initiative KEYWORDS * _in-situ_ * hybridization * karyotypes * _Lathyrus_ * silver staining