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ABSTRACT Type II DNA topoisomerases catalyse DNA double-strand cleavage, passage and re-ligation to effect topological changes. There is considerable interest in elucidating topoisomerase II

roles, particularly as these proteins are targets for anti-cancer drugs. Here we uncover a role for topoisomerase IIα in RNA polymerase I-directed ribosomal RNA gene transcription, which

drives cell growth and proliferation and is upregulated in cancer cells. Our data suggest that topoisomerase IIα is a component of the initiation-competent RNA polymerase Iβ complex and

interacts directly with RNA polymerase I-associated transcription factor RRN3, which targets the polymerase to promoter-bound SL1 in pre-initiation complex formation. In cells, activation of

rDNA transcription is reduced by inhibition or depletion of topoisomerase II, and this is accompanied by reduced transient double-strand DNA cleavage in the rDNA-promoter region and reduced

pre-initiation complex formation. We propose that topoisomerase IIα functions in RNA polymerase I transcription to produce topological changes at the rDNA promoter that facilitate efficient

_de novo_ pre-initiation complex formation. SIMILAR CONTENT BEING VIEWED BY OTHERS TDP1 SUPPRESSES CHROMOSOMAL TRANSLOCATIONS AND CELL DEATH INDUCED BY ABORTIVE TOP1 ACTIVITY DURING GENE

TRANSCRIPTION Article Open access 09 November 2023 TRANSCRIPTIONAL REPRESSION BY A SECONDARY DNA BINDING SURFACE OF DNA TOPOISOMERASE I SAFEGUARDS AGAINST HYPERTRANSCRIPTION Article Open

access 13 October 2023 POLY(ADP-RIBOSYLATION) OF P-TEFB BY PARP1 DISRUPTS PHASE SEPARATION TO INHIBIT GLOBAL TRANSCRIPTION AFTER DNA DAMAGE Article 07 April 2022 INTRODUCTION Topoisomerases

cleave DNA to elicit topological changes, facilitating DNA-processing events in cells, including transcription1,2,3. The type II topoisomerases (Top2) relax supercoiled DNA by a

double-strand DNA passage reaction. There is much interest in understanding the cellular roles of the Top2 enzymes, the mechanisms and sites of action and the processes involved in

recruitment to these sites, particularly as these proteins are targets for clinically important anti-cancer drugs4,5,6. In transcription, Top2 activity has been implicated in resolving

supercoiling associated with elongation by RNA polymerases7,8,9,10,11,12. In RNA polymerase I (Pol I) transcription, in yeast, Top2 cleavage resolves the positive supercoiling ahead of the

elongating polymerase, whereas Top1 resolves negative torsion behind the polymerase7 and, in mammalian cells, Top1 has been shown to have an important role in Pol I transcription

elongation13,14,15. Mammalian cells have two isoforms of Top2, α and β, with similar enzymatic activities and 68% overall sequence identity, but Top2α and β differ markedly in their

C-terminal domains (CTDs), which appear to determine isoform-specific functions. Top2α, specifically, is essential for chromatid segregation and decatenation G2-checkpoint function16,17, for

instance, whereas, Top2β is involved in the repair of DNA cross-links and the transcriptional induction of a subset of hormone- and developmentally regulated genes in Pol II

transcription18,19,20,21,22. To our knowledge, a Top2α-specific role in transcription has not yet been described. Intriguingly, our proteomic analyses of Pol I complexes had revealed,

previously, the specific co-purification of Top2α with the initiation-competent Pol Iβ complex23. Pol I transcription produces the major ribosomal RNA (rRNA) constituents of the

protein-synthesis machinery, driving cell growth and proliferation and, thereby, influencing cell fate24,25. Upregulation of Pol I transcription is linked to the unrestrained growth and

proliferation characteristic of cancer cells26,27. Here we present evidence for a role for Top2α in the early stages of the Pol I transcription cycle. We demonstrate that Top2α is a

component of Pol Iβ and can bind to the RRN3 component of Pol Iβ, which bridges the interaction between Pol I and basal transcription factor SL1 at the rRNA gene promoter28,29,30. We found

that drug-induced inhibition of Top2 activity did not prevent elongation of rRNA transcripts. Our data suggest a novel and specific role for Top2α activity in facilitating _de novo_

pre-initiation complex (PIC) formation in rRNA gene transcription. Top2 inhibitors produced a defect in activation of Pol I transcription, independently of the DNA-damage response pathways,

suggesting that drugs designed to target Top2α in Pol I transcription could be useful non-genotoxic agents in the treatment of cancer. RESULTS ACTIVE TOP2Α IS A COMPONENT OF

INITIATION-COMPETENT POL IΒ Pol I transcribes the rRNA gene repeats to produce the 47S pre-rRNA transcript that is processed into the 18S, 5.8S and 28S rRNAs24,25,28,31. Two functionally

distinct forms of Pol I complex can be extracted from the nucleus of human cells. The Pol Iα complex, the most abundant form of Pol I in nuclear extracts, is catalytically active but does

not support promoter-specific initiation at an rRNA gene promoter. The Pol Iβ complex accounts for ~10% of Pol I activity and is competent for promoter-specific transcription initiation. Pol

Iβ is defined by the association of its Pol I core subunits with growth-regulated transcription initiation factor RRN3, which bridges the interaction between basal transcription factor SL1

and Pol I in formation of functional PICs at the rRNA gene promoter24,25,28. We have previously reported that Top2α co-fractionates with the Pol Iβ promoter-specific transcription activity

and is the major substrate for Pol Iβ-associated CK2 in the Pol Iβ complex23. We demonstrate that Pol Iβ has an associated decatenation activity that is ATP-dependent and sensitive to

non-hydrolysable ATP and Top2 inhibitor etoposide (Fig. 1a). The decatenation activity and Top2α protein co-purify with the RRN3 component of Pol Iβ (Supplementary Fig. S1a). These data

suggest that catalytically active Top2α is associated with the initiation-competent Pol Iβ complex. To substantiate an association of Top2α with Pol Iβ in cells, we immunoprecipitated Pol I

from nuclear extracts of HeLa cells transiently expressing Flag-tagged Pol I subunit CAST32, using Flag-specific antibodies, then immunoblotted the immunoprecipitates using antibodies

specific for Top2α, RRN3 and Pol I subunits PAF53 and AC19. Top2α co-immunoprecipitated with Pol I in this experiment (Fig. 1b). We also passed purified Pol Iβ over a Top2α-antibody column

and analysed proteins eluted from the column and proteins in the flow-through in immunoblot and promoter-specific transcription assays (Supplementary Fig. S1b–d). Intriguingly, the majority

of promoter-specific transcription activity was lost and most of the RRN3 protein was depleted from Pol Iβ and retained on the Top2α-antibody column. Therefore, the specific Top2α antibody

used disrupted Top2α and RRN3 interactions with Pol I. These data indicate that the majority of Pol Iβ complexes purified from human cell nuclei include Top2α. TOP2Α INTERACTS DIRECTLY WITH

POL IΒ-SPECIFIC COMPONENT RRN3 We reasoned that a direct interaction between Top2α and Pol Iβ might involve Pol Iβ-specific component RRN3. Two polypeptides unique to Pol Iβ bound to RRN3,

the larger of which migrated similarly to full-length Top2α protein, in a Far-western analysis in which _in vitro_-translated 35S-labelled hRRN3 was used to probe renatured Pol Iα or Pol Iβ

(Fig. 1c). Moreover, there was a direct interaction between RRN3 and Top2α in a recombinant protein-binding assay, in which _in vitro_-translated RRN3 was incubated with _in

vitro_-translated green fluorescent protein (GFP)-Top2α33 on GFP-antibody beads (Fig. 1d). To test the likely involvement of the isoform-specific CTD in the interaction of Top2α with RRN3,

we used Top2α CTD-mutant proteins lacking the terminal 42 or 180 amino acids (st1 or st5, respectively). These were the shortest and longest of a series of CTD truncations shown to impair

the ability of GFP-Top2α to rescue the conditional Top2α-mutant cell line HTETOP33 from lethal Top2α depletion (Supplementary Fig. S2). A C-terminal-truncated version of Top2α (st5, 180

amino-acid deletion) displayed significantly reduced interaction with RRN3 in the recombinant protein-binding assay (Fig. 1d). To further assess Top2α–RRN3 interactions, nuclear extracts of

HTETOP cells depleted of endogenous Top2α33 and stably expressing GFP-Top2α-wild type (WT) or -st1 (CTD 42 amino-acid deletion) were incubated with GFP-specific antibodies, and

immunoprecipitates were immunoblotted using antibodies specific for Top2α, RRN3 and Pol I subunit PAF53. Endogenous RRN3 and Pol I subunit PAF53 co-immunoprecipitated with GFP-Top2α-WT (Fig.

1e). There was a significant decrease (~sixfold) in the amount of RRN3 that co-immunoprecipitated with the GFP-Top2α-st1 mutant, compared with GFP-Top2α-WT. There was also a decrease

(~twofold) in the amount of PAF53 co-immunoprecipitated; the smaller magnitude of this decrease might reflect interactions between Top2α and other components of the initiation-competent Pol

Iβ complex, such as CK223. Collectively, these data demonstrate that Top2α interacts directly with RRN3 and that residues 1491-1531 in the Top2α CTD are important for such interaction.

Therefore, there is evidence, both _in vitro_ and in cells, to support an interaction between Top2α and RRN3 as components of initiation-competent Pol Iβ. TOP2Α OCCUPIES THE RDNA PROMOTER IN

AN SL1-DEPENDENT MANNER The association of Top2α with initiation-competent Pol Iβ predicts the presence of Top2α at the rDNA promoter. Top2α was detectable at the rDNA promoter by chromatin

immunoprecipitation (ChIP) analysis in all cell types tested (Fig. 1f and Supplementary Fig. S3); elsewhere, along the rDNA repeat, Top2α association varied according to cell type

(Supplementary Fig. S3). Small interfering RNA-mediated depletion of the TAFI41 subunit of SL1 in cells, which leads to the disappearance of SL1 and Pol I from the rDNA promoter and reduces

Pol I transcription34, resulted in the loss of Top2α from the rDNA promoter (Fig. 1f and g). The data suggest that SL1, which binds to the rDNA promoter and recruits Pol Iβ through RRN3, is

required for the recruitment of Top2α to the rDNA promoter in cells and that the presence of Top2α at the rDNA promoter correlates with the presence of Pol Iβ and Pol I transcription. TOP2Α

AND RRN3 DISSOCIATE FROM POL I FOLLOWING INITIATION RRN3 dissociates from Pol I at an early step following initiation of transcription35,36, and we have used a stalled Pol I transcription

system37 to assess whether Top2α and RRN3 both dissociate from Pol I following initiation of transcription. Pol I can be stalled at the position of the first T (+31) on a ‘T-less’ template

when the transcription reaction is carried out in the absence of UTP. Immobilization of the template allows the template-associated proteins to be separated from proteins that dissociate

from the transcription complex after initiation of transcription. We demonstrate that Pol I subunit PAF53 was still associated with the DNA template following initiation of transcription, as

expected for a stalled Pol I complex (Fig. 2a, lane 1), whereas RRN3 and Top2α were present in the reaction supernatant (Fig. 2a, lane 2). Note that in control transcription reactions

supplemented with all four NTPs, Pol I transcribes to the end of the template, whereupon it dissociates in the presence of excess competitor DNA (Fig. 2a, lane 3) and, consequently, Pol I

and Pol I-associated factors were present in the reaction supernatant (Fig. 2a, lane 4). Therefore, both Top2α and RRN3 dissociate from Pol I following initiation of transcription _in vitro_

(Fig. 2b), consistent with a tethering of Top2α to Pol I, at least in part, through RRN3. Should Top2α indeed dissociate from Pol I at initiation or during/immediately following promoter

escape in cells, any role for Pol Iβ-associated Top2α in Pol I transcription would be predicted to be at an early step in transcription. EFFECTS OF TOP2 INHIBITION ON POL I TRANSCRIPTION To

investigate a role for Top2α in early Pol I transcription events, we first tested the effects of inhibition of Top2 activity on Pol I transcription, using 3H-uridine pulse-chase labelling of

(pre-)rRNA in cells. We observed a negative effect on Pol I transcription of etoposide treatment of U2OS cells (Supplementary Fig. S4). However, there is evidence to suggest that this was

likely to be indirect and as a consequence of ataxia telangiectasia mutated (ATM)-38 and p53-dependent DNA-damage signaling39,40, activated by double-strand DNA breaks arising from trapped

Top2–DNA cleavage complexes1. Therefore, to assess whether inhibition of Top2 activity could induce any effects on Pol I transcription independent of DNA-damage signalling, we treated cells

with alternative Top2 inhibitors and/or used cell lines that could not elicit a p53-dependent DNA-damage response. Crucially, treatment for up to 15 h of U2OS cells with merbarone (a Top2

catalytic inhibitor blocking DNA cleavage41) and of p53-null H1299 cells with etoposide (Fig. 3a–c) produced no significant effects on Pol I transcription. As inhibition of Top2 activity for

up to 15 h does not have a detectable direct effect on Pol I transcription in actively growing cell populations, this suggests that Top2 activity is not essential for transcription

(re-)initiation or elongation of rRNA transcripts. Notably, Top2 inhibitor treatments for 24 and 48 h, of U2OS cells with merbarone or HCT116 (p53 null) cells with etoposide, resulted in

significant decreases in Pol I transcription (Fig. 3d–f). These findings imply a potential role for Top2 in Pol I transcription, outside of (re-)initiation or elongation. TOP2Α DEPLETION

NEGATIVELY AFFECTS POL IΒ ASSEMBLY/STABILITY To further explore the possibility of a role for Top2α in Pol I transcription, we analysed rRNA transcripts from HTETOP cells specifically

depleted of the α-isoform of Top2 by treatment with tetracycline (Tet) for 48 h (Fig. 4a and b). In common with other cells depleted of Top2α protein or treated with Top2 catalytic

inhibitors (reviewed in Nitiss1), this impairs sister chromatid segregation causing aberrant anaphases and cytokinesis16,33. After 48 h in Tet, the only mRNA transcripts to be dramatically

depleted in HTETOP cells are those encoding Top2α itself42. Nevertheless, we detected an ~twofold reduction in Pol I synthesis of the 47S pre-rRNA transcript, with no effect on pre-rRNA

processing (Fig. 4c). Pol I was immunoprecipitated from the Top2α-depleted and control cells in equivalent amounts, as determined by the non-specific Pol I transcription activities of the

immunoprecipitates (Fig. 4d). Yet, there was significantly less promoter-specific transcription activity associated with Pol I immunoprecipitates from the Top2α-depleted cells (Fig. 4d) and

a reduced amount of RRN3 protein in these immunoprecipitates (Fig. 4e), compared with those of the control cells. These data suggest the presence of fewer initiation-competent Pol Iβ

complexes in Top2α-depleted cells. Such a decrease could account for the observed two-fold reduction in Pol I transcription in Top2α-depleted cells. Taken together, these results suggest

that Top2α can influence the assembly and/or stability of initiation-competent Pol Iβ at the rDNA promoter, and thereby PIC formation, in cells. We reasoned that in a population of actively

growing cells, at any one time, most of the active rDNA promoters are engaged in multiple-round transcription, with relatively few requiring _de novo_ PIC formation and activation of

transcription. _De novo_ PIC formation is required at actively transcribing rDNA genes following DNA replication (on one set of duplicates). Lack of _de novo_ PIC assembly would lead to a

predicted ~50% reduction in Pol I transcription with each cell cycle. Our data (Figs 3 and 4) suggest that in the absence of Top2α activity, there may be a gradual accumulation of rDNA

promoters requiring _de novo_ PIC formation to achieve transcription. TOP2Α FACILITATES ASSEMBLY OF POL I PICS To investigate the involvement of Top2α in _de novo_ PIC formation, we sought a

system in which _de novo_ functional PIC formation was required for Pol I transcription at the majority of rDNA promoters. Pol I transcription can be downregulated by serum starvation of

cells and activated by serum refeeding43,44. Starved U2OS cells exhibit decreased levels of Pol I transcription (Fig. 5a), accompanied by reduction of SL1 and Pol I from the rDNA promoter

(Fig. 5b) and the disappearance of Top2α from the rDNA promoter (as determined by ChIP) (Fig. 5b) and the nucleolus (as determined by indirect immunofluorescence; Supplementary Fig. S5).

Activation of Pol I transcription occurs in starved H1299, U2OS and HTETOP cells following serum refeeding (Fig. 6a–c, respectively). This activation correlates with an increase in SL1, UBF

and Pol I promoter occupancies and the reappearance of Top2α at the rDNA promoter (Fig. 6d), as well as increased Top2α expression and co-localisation of Top2α with Pol I in the nucleolus

(Fig. 6f and Supplementary Fig. S5b). Therefore, _de novo_ functional PIC formation appears to be required for transcription at the majority of rDNA promoters re-activated in serum-refed

cells. There are several hundred copies of the rRNA genes per cell, but only about half are active in cycling cells and sensitive to psoralen cross-linking, and these are in an open

chromatin configuration. Despite the changes in Pol I transcription caused by starvation or growth-stimulation of cells, the proportion of rRNA genes in an open chromatin configuration

remains constant43,45. This suggests that the rRNA genes requiring _de novo_ PIC formation for activation upon serum refeeding are likely to be from the pool of genes sensitive to psoralen

cross-linking. We exploited this system to investigate the requirement for Top2α in _de novo_ PIC formation in Pol I transcription. There was a marked (~twofold) reduction in activation of

Pol I transcription in starved H1299 and U2OS cells treated with Top2 inhibitors etoposide and merbarone, respectively, then resupplied with serum, as determined by S1 nuclease protection of

the first 40 nucleotides of the pre-rRNA (Fig. 6a) and by 3H-uridine pulse-chase labelling (Supplementary Fig. S6a,b). Top2 inhibitor ICRF-193 similarly reduced activation of Pol I

transcription in U2OS cells (Supplementary Fig. S6d,e). These data suggested a defect in the early stages of transcription. There was a corresponding reduced occupancy of SL1, UBF and Pol I,

with little Top2α detectable, at the rDNA promoter in the H1299 cells (Fig. 6d), suggesting that, inhibition of Top2 activity reduces the efficiency of PIC formation and, thereby, the

efficiency of Pol I transcription activation. This defect in activation is likely to be independent of DNA-damage signalling through p53 and ATM as it occurred in p53-null cells (H1299)

(Fig. 6a) and could not be rescued by incubation of cells with caffeine (an inhibitor of ATM/ATR [ataxia telangiectasia and Rad3-related] signalling) (Fig. 6g). HTETOP cells depleted of

Top2α protein, serum-starved, then resupplied with serum, also showed reduced activation of Pol I transcription and a corresponding reduced promoter occupancy of SL1, UBF and Pol I (Fig. 6c

and Supplementary Fig. S6c), complementing the results obtained by pharmacological inhibition of Top2. Taken together, these results suggest that Top2 activity, specifically that of the

α-isoform of Top2, facilitates the efficient _de novo_ assembly of PICs in Pol I transcription. TOP2Α INDUCES DNA CLEAVAGE AT THE RDNA PROMOTER IN ACTIVATION We hypothesized that Top2α might

influence _de novo_ PIC formation in activation of Pol I transcription by producing topological changes at the rDNA promoter that would support efficient assembly of the PIC. We reasoned

that double-strand DNA (dsDNA) cleavage would arise at the rDNA promoter from such Top2 activity, although short-lived, due to the re-ligation activity of Top2. Therefore, we analysed the

rDNA-promoter region for DNA breaks. To this end, cells were fixed and permeabilized at different time points following serum refeeding, incubated with biotin-11–deoxyuridine triphosphate

(dUTP) and deoxynucleotide transferase (TdT) to label DNA breaks, and then, DNA containing breaks was captured on streptavidin beads and analysed by qPCR, as described19. Strikingly, a

significant increase in DNA cleavage at the rDNA promoter was detectable during the activation of Pol I transcription (Fig. 7a). The time course demonstrates that the DNA cleavage is

transient, peaking at the first detection of transcription activation and Top2α occupancy of the rDNA promoter. Importantly, no such DNA cleavage was detectable at the rDNA promoter in the

Top2α-depleted and starved HTETOP cells upon serum refeeding (Fig. 7b). Note that although enhanced DNA cleavage has been observed at other regions of the rDNA in some experiments upon serum

refeeding and Pol I transcription activation, this was not a reproducible effect. In other control experiments, we did not observe any increase in DNA cleavage at the promoters of the

glyceraldehyde-3-phosphate dehydrogenase and peptidylprolyl isomerase A genes upon serum refeeding (Supplementary Fig. S7). Our data imply that, in activation of Pol I transcription, Top2α,

specifically, induces the transient appearance of double-strand DNA breaks in the rDNA-promoter region, reflecting its dsDNA cleavage, strand passage and re-ligation activity. Taken

together, the data suggest that Top2α activity at the rDNA promoter facilitates the efficient _de novo_ assembly of functional PICs, which include SL1, UBF and Pol Iβ (Fig. 7c). DISCUSSION

This study identifies a novel function for a Top2 in facilitating _de novo_ PIC formation and activation of Pol I transcription of the rRNA genes in human cells. We present evidence of a

role for the Top2α isoform in Pol I transcription. Our data suggest that active Top2α is a component of the initiation-competent Pol Iβ complex, targeted to the rDNA promoter, at least in

part, through the interaction of its isoform-specific C terminus with the RRN3 component of Pol Iβ, which interacts with promoter-bound transcription factor SL1. Depletion of Top2α

negatively affects the assembly and/or stability of initiation-competent Pol Iβ and decreases Pol I transcription in cells, implying that Top2α can influence the assembly and/or stability of

initiation-competent Pol Iβ at the rDNA promoter and, thereby, PIC formation in cells. _De novo_ PIC formation is an event expected to occur at the active rDNA gene promoters following DNA

replication (on one set of the duplicates) during each cell cycle. _De novo_ functional PIC formation is also required for activation of Pol I transcription at the majority of rDNA promoters

upon refeeding of serum-starved cells, and we discovered that Top2α facilitates efficient activation of Pol I transcription from such promoters and that this is accompanied by

Top2α-dependent DNA cleavage and accumulation of PIC components and Top2α at the rDNA-promoter region. Our data suggest a role for Top2α in _de novo_ PIC formation, and we propose that Top2α

facilitates efficient activation of Pol I transcription through its ability to cleave, passage and re-ligate dsDNA and, thereby, to alter the topology of the rDNA promoter, alleviating

topological constraints to PIC assembly and stability (Fig. 7c). At the rDNA promoter, the local topology or higher-order structure46,47 of the DNA can influence transcription of the rRNA

gene and can be affected by chromatin context, including binding of the architectural protein UBF, which bends and supercoils the promoter48,49, and nucleosome positioning50. TBP–TAF complex

SL1 directs Pol I PIC formation and stabilizes UBF at the rDNA promoter51. We envisage that, in activation of Pol I transcription, SL1 binds to the rDNA promoter, RRN3 binds to SL1 and

Top2α is recruited through its interaction with RRN3, and then Top2α-mediated cleavage, passage and re-ligation of dsDNA at the rDNA promoter creates a topological state conducive to the

efficient _de novo_ assembly and stabilization of a functional PIC, including SL1, UBF and initiation-competent Pol Iβ, so that transcription can now be initiated and re-initiated. Lack of

Top2α catalytic activity during _de novo_ PIC formation would reduce Pol I transcription activation by affecting the equilibrium of SL1 and UBF binding to the promoter and, thereby, the

efficiency of Pol I recruitment. Top2α could also, potentially, facilitate efficient _de novo_ PIC formation upon activation of rDNA transcription by stimulating promoter escape and Pol I

processivity in the pioneering round of transcription. In actively growing cells, a relatively high density of Pol I complexes facilitates Pol I clustering52. Consequently, positive

supercoils ahead of the transcribing complex and negative supercoils behind7,12 could potentially be dissipated by the actions of the adjacent polymerases52, such that topoisomerase activity

would not be required, except perhaps ahead of a stalled polymerase or in regions where the density of polymerases is sparse. A polymerase pioneering the first round of activated

transcription, without the advantage of an adjacent polymerase to dissipate the supercoiling it generates as it transcribes the rDNA would require topoisomerase cleavage. In the absence of

Top2α, the observed decrease in occupancy by PIC components of the rDNA promoter might be accounted for if pioneering polymerases were stalled, due to the topological constraints imposed by

failure to resolve supercoiling at or before promoter escape, thereby preventing the productive interaction of incoming PIC components with the rDNA promoter. We considered the possibility

that Top2α might affect serum-activated _de novo_ PIC formation by influencing the assembly of nucleosome remodelling machineries for repositioning of nucleosomes through a mechanism similar

to that proposed for DNA topoisomerase IIβ (Top2β) in ligand (hormone)-stimulated activation of Pol II promoters19, which involves recruitment of DNA-damage response proteins. However, such

a mode of action seems unlikely, as key components of the repair machineries (such as Ku70, Ku80 and DNA-PK) were not detectable by ChIP analysis at the activated rDNA promoters (our

unpublished results), suggesting that, if Top2α affects nucleosome positioning in activation of Pol I transcription, then it achieves this through alternative means. Our findings reveal a

novel dimension to the efficacy of Top2 inhibitors used in cancer treatment4,5,6 and, potentially, to the search for Top2α-specific anti-cancer agents5,53. _De novo_ PIC formation and

activation of Pol I transcription occur during each cell cycle at newly replicated rRNA genes and might also be required for the upregulation of Pol I transcription linked to cancer26,27. We

have demonstrated that the Top2 inhibitor etoposide, an effective anti-cancer drug, can reduce _de novo_ PIC assembly and activation of Pol I transcription, independently of the p53 status

of cells and the ATM/ATR-dependent DNA-damage response pathways. This suggests that this Top2 inhibitor might function in part to restrict Pol I transcription by limiting _de novo_

activation of rRNA genes, which, ultimately, could lead to the abrogation of Pol I transcription, even in p53-null cells. This would have devastating consequences for protein synthesis,

constraining the runaway growth associated with cancers. Indeed, maintenance of elevated levels of Pol I activity in cancer cells appears critically important for the process of malignant

transformation and cancer cell survival. For instance, CX-5461, a selective inhibitor of Pol I transcription, induced p53-dependent apoptotic cell death in the majority of Eμ-Myc lymphoma

cells at concentrations that reduced Pol I transcription about 50% (ref. 54). Recent studies have illustrated the effectiveness of targeting Pol I transcription in anti-cancer therapy for

haematological malignancies54 and solid tumours55. Therefore, we speculate that inhibitors specifically designed to target Top2α in Pol I transcription (which may be less likely to cause

secondary cancers than those targeting the β-isoform53) could be effective non-genotoxic tools for use in the battle against cancer. METHODS CELL-CULTURE CONDITIONS AND TOP2 DEPLETION OR

INHIBITION U2OS cells in McCoy’s 5A medium plus 10% FBS, H1299 cells (homozygous partial deletion of p53) in RPMI plus 10% FBS and HTETOP cells (derivative of human fibrosarcoma cell line

HT1080) in DMEM high glucose (4.5 g l−1) plus 10% FBS and other additives33 were grown to ~60–70% confluency, washed twice with Dulbecco’s PBS and then serum-starved for 20 h in DMEM low

glucose (1 g l−1). For activation of Pol I transcription, serum-starved cells were incubated in DMEM low glucose (1 g l−1) containing 20% FBS. For Top2 inhibition, Top2 poison etoposide (100

 μM final concentration; Merck) or catalytic inhibitors ICRF-193 (50 μM) and merbarone (100 μM; Merck) were added (except Fig. 6g). For Top2α depletion, HTETOP medium was supplemented with 1

 μg ml−1 Tet for 48 h. CELL AND _IN VITRO_ EXPRESSION OF GFP-TOP2Α FUSION PROTEINS HTETOP cells expressing GFP-Top2α were as described (Clone H33). Stop codons were introduced into

pGFP-Top2α33 by Quickchange site-directed mutagenesis (Agilent Technologies; see Supplementary Fig. S2). pGFP-Top2α-WT (full-length human; 1,531 amino acids) or pGFP-Top2α-st1 (stop-codon

mutant) plasmids33 (_Not_I-linearized) were electroporated into HTETOP cells, puromycin-resistant colonies were selected and single GFP-positive colonies were expanded. _In vitro_

transcription/translation experiments used pGFP-Top2α-WT and -st5. IMMUNOCYTOCHEMISTRY Cells were fixed (10 min, 4% paraformaldehyde), permeabilized (10 min, 1% Triton X-100) and blocked (10

 min, 1% donkey serum in PBS), and then incubated (1 h) with antibodies (Supplementary Table S1) in the blocking buffer, washed X3 for 10 min in PBS and incubated (1 h) with labelled

secondary antibodies (Supplementary Table S1). After washes, cells were mounted with Vectashield containing 4′,6-diamidino-2-phenylindole and visualized using confocal microscopy. RNA

LABELLING IN CELLS AND RRNA ANALYSIS Labelling of RNA in cells (~70% confluent or ~50% for starved/refed) involved 10 μCi 3H-uridine for ~0.2–0.4 × 105 cells per well of a six-well plate. In

pulse-chase labelling, cells were incubated for 2 h with 3H-uridine, washed and incubated in unlabelled medium containing 0.5 mM uridine (+/− Top2 inhibitors). RNA was extracted (RNeasy

Mini Kit (Qiagen)). An amount of 2 μg of 3H-labelled total RNA was run on a 1% formaldehyde agarose gel at 130 V for 90 min in X1 MOPS, blotted onto Hybond-N membrane (Amersham),

cross-linked (ultraviolet cross-linker; UVP), analysed by tritium imaging using Fuji Tritium image plate (or following PerkinElmer En3Hance spray, exposed to Kodak Biomax XAR film at −80 °C)

and then quantified using Aida software. S1 NUCLEASE PROTECTION ANALYSIS OF PRE-RRNA LEVELS Total RNA isolation from cells (~70% confluent or ~50% for starved/refed) and S1 nuclease

protection analysis were performed with a 5′ end-labelled oligonucleotide probe complementary to the first 40 nucleotides of the 47S pre-rRNA transcript44. _IN VITRO_ TRANSCRIPTION ASSAYS

Non-specific transcription assays were performed as described28. Run-off transcription reactions were performed essentially as described37, using immobilized rDNA fragments containing the

human rRNA gene promoter, supplemented with purified SL1 and recombinant UBF56. Reactions were terminated by addition of RTL buffer and RNA transcripts (purified using RNeasy mini kits

(Qiagen)) were electrophoresed on denaturing 4% acrylamide/8 M urea gels, visualized by phosphorimaging FLA-7000 (Fuji) and analysed with Aida software. DECATENATION ASSAY DNA topoisomerase

II activity was assayed by analysing decatenation of kinetoplast DNA (TopoGen), according to the manufacturer’s protocols. AMP-PNP was substituted for ATP (200 and 100 μM) or Top2 inhibitor

etoposide (250 and 100 μM) was included in some reactions. DNA products were resolved on a 1% agarose gel in TBE buffer. IMMUNOBLOTTING AND IMMUNOPRECIPITATION Antibodies used for

immunoblotting and immunoprecipitations (IPs) are listed (Supplementary Table S1). IP of nuclear extracts, prepared as described57, from U2OS and HTETOP cells (±1 μg ml−1 Tet) transfected

(FuGENE HD reagent; Roche) with Flag-CAST expression vector pcFCAST32 used anti-Flag M2 magnetic beads (Sigma) (for Pol I IP) for 2 h at 4 °C. The beads were washed three times in TM10/0.15 

M KCl (TM10 buffer: 50 mM Tris-HCl pH 7.9, 12.5 mM MgCl2, 1 mM EDTA, 10% glycerol, 1 mM sodium metabisulphite and 1 mM dithiothreitol). Washed precipitates were analysed by immunoblotting.

IP of nuclear extracts from HTETOP GFP-Top2α cells used anti-GFP-antibody magnetic beads (for Top2α IP). PROTEIN–PROTEIN INTERACTION ASSAYS Far-western analysis was performed as described28.

Pol Iα and β fractions were subjected to SDS–PAGE and transferred to an Immobilon-P (Millipore) membrane. After renaturation, the membrane was probed with FLAG-purified,

35S-methionine-labelled hRRN3 and human Top2α antibody. Interactions of GFP-tagged Top2α-WT and Top2α-st5 with Flag-tagged human RRN3 were analysed using 35S-labelled _in vitro-_translated

proteins (TNT-coupled reticulocyte lysate; Promega) in _in vitro_ binding assays. Proteins were immunoprecipitated from the labelling reactions using anti-GFP, anti-FLAG and anti-HA antibody

magnetic beads. Human RRN3 was eluted from washed beads using Flag-peptides and incubated with GFP-Top2α-WT/-st5 on beads. After binding (75 mM KCl in TM10 buffer with 0.015% NP40 and 1 μg 

ml−1 BSA) for 30 min on ice, beads were washed (0.1 M KCl in TM10) and bound proteins were analysed by SDS–PAGE and autoradiography. CHIP ASSAY Cells were subjected to cross-linking (1%

formaldehyde, room temperature, 10 min; terminated by addition of glycine to a final concentration of 0.125 M for 5 min) and then chromatin was isolated as described58 and sheered to ~300 bp

of average-size fragments (Bioruptor; Diagenode). A single ChIP used chromatin from 2.5 × 105 or 1 × 106 cells and antibodies as in Supplementary Table S2, incubated overnight at 4 °C, and

then for 2 h with Protein A/G beads (Invitrogen) at room temperature. Beads were washed five times in 450 μl volume of RIPA ChIP buffer59 and twice with 200 μl TE buffer with aid of the

Precipitor (Abnova). DNA on washed beads59 was eluted, and cross-link reversal was performed in one stage as described60. DNA was purified (IPure kit; Diagenode with Precipitor; Abnova) and

analysed by qPCR (QuantiFast Multiplex PCR Mix; Qiagen) in triplicates using primer combinations and probes covering regions of rDNA repeat (Supplementary Table S3) on Light Cycler 480-II

(Roche). Results were expressed as percentage of input chromatin and normalized to control IgG levels. Bar graphs show the average from three independent experiments (_n_=3); s.d. and

statistical significance (probability values: ***_P_<0.001; **_P_<0.01; and *_P_<0.05, for drug-treated versus control cells) have been calculated using one- and two-way analysis of

variance on R software (open-source statistical computing and graphics software). DNA DOUBLE-STRAND BREAK DETECTION ASSAY Cells were fixed, permeabilized _in situ_ and DNA breaks were

labelled with biotin-11–dUTP and terminal TdT as described19. In brief, cells (15 cm dish per single experimental point) were washed with 37 °C PBS and fixed for 20 min with Streck Fixative

(0.15 M 2-bromo-2-nitropropane-1,3-diol, 0.1 M diazolidinyl urea, 0.04 M zinc sulphate heptahydrate, 0.01 M sodium citrate dihydrate and 50 mM EDTA) at room temperature and permeabilized by

further incubation for 15 min at room temperature in PM buffer (0.1 M Tris-HCl, pH 7.5, 50 mM EDTA and 1% Triton X-100). Cells were washed six times with 10 ml of water before one wash with

3 ml of X1 TdT buffer containing 0.005% Triton X-100. Cells were subsequently incubated in 4 ml reaction buffer (X1 TdT buffer, 0.005% Triton X-100, 45 μM biotin-11–dUTP (Fermentas) and 250 

U ml−1 terminal deoxynucleotidetransferase (Promega)) for 90 min at 37 °C on rotating platform. Cells were washed twice with 10 ml ice-cold TBS (50 mM Tris-HCl pH 7.5, 150 mM NaCl) and then

carefully collected with a large cell scraper, pelleted by centrifugation and lysed in 200 μl lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA and 2% SDS) for 15 min at 37 °C. Chromatin was

sheared using the Bioruptor (Diagenode) to 500 bp of average-size fragments, and diluted and biotinylated DNA fragments were captured with 50 μl of Streptavidin M280 magnetic beads

(Invitrogen) for 2 h at room temperature. Beads were washed five times in 450 μl of RIPA ChIP buffer59 and twice in 200 μl TE buffer with the aid of a Precipitor (Abnova). DNA elution was

performed as described60. Purified DNA was analysed by qPCR using QuantiFast Multiplex PCR Mix (Qiagen) and the rDNA promoter-specific combination of primers and probe (see Supplementary

Table S3) on Light Cycler 480-II (Roche). For the glyceraldehyde-3-phosphate dehydrogenase promoter control, we used the following forward and reverse primers: 5′-CAACGGATTTGGTCGTATTGG-3′

and 5′-TGATGGCAACAATATCCACTTTACC-3′ with the probe 5′-TCACCAGGGCTGCTT-3′ (Applied Biosystems). For the peptidylprolyl isomerase A gene promoter, the primers were as follows:

5′-CAAATGGTTCCCAGTTTTTCATC-3′ and 5′-TTGCCAAACACCACATGCTT-3′ with the probe 5′-CACTGCCAAGACTG-3′ (Applied Biosystems). PCR parameters were set as recommended by PCR Mix manufacturer

(Qiagen). All PCR reactions were performed in triplicate and averages; s.d. and statistical significance (***_P_<0.001; **_P_<0.01; *_P_<0.05; by analysis of variance) were derived

from three independent experiments (_n_=3). Results were expressed as percentage of input chromatin and normalized to control reactions where no biotin-11–dUTP was added at the labelling

stage. ADDITIONAL INFORMATION HOW TO CITE THIS ARTICLE: Ray, S. _et al._ Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex

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ACKNOWLEDGEMENTS We thank R. Hannan (Peter MacCallum Cancer Centre, Melbourne) for UBF antibodies, S. Church (Queen’s University Belfast) for his help with microscopy and all members of the

K.I.P. and J.C.B.M.Z. Laboratories for their help. We also thank D. McCance, D. Timson (both at Queen’s University Belfast), T. Owen-Hughes and S. Goodfellow (both at University of Dundee)

for their critical reading of the manuscript and helpful suggestions. This work was supported by Cancer Research UK Grant (C9700/A5962) to A.C.G.P., an MRC New Investigator Award to K.I.P.

(Grant ID: 89365) and a Wellcome Trust Programme Grant to J.C.B.M.Z. (085441/Z/08/Z). AUTHOR INFORMATION Author notes * Konstantin I. Panov and Joost C. B. M. Zomerdijk: These authors

contributed equally to this work AUTHORS AND AFFILIATIONS * School of Biological Sciences and the Centre for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast, BT9 7BL,

UK Swagat Ray, Tatiana Panova & Konstantin I. Panov * Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK Tatiana Panova, Gail

Miller, Jackie Russell, Konstantin I. Panov & Joost C. B. M. Zomerdijk * Gene Targeting Group, Centre for Haematology, Imperial College Faculty of Medicine, Du Cane Road, London W12 0NN,

UK, Arsen Volkov & Andrew C. G. Porter Authors * Swagat Ray View author publications You can also search for this author inPubMed Google Scholar * Tatiana Panova View author

publications You can also search for this author inPubMed Google Scholar * Gail Miller View author publications You can also search for this author inPubMed Google Scholar * Arsen Volkov

View author publications You can also search for this author inPubMed Google Scholar * Andrew C. G. Porter View author publications You can also search for this author inPubMed Google

Scholar * Jackie Russell View author publications You can also search for this author inPubMed Google Scholar * Konstantin I. Panov View author publications You can also search for this

author inPubMed Google Scholar * Joost C. B. M. Zomerdijk View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS S.R., K.I.P., T.P. and G.M.

carried out the experiments. A.V. and A.C.G.P. constructed the conditional Top2α expressing cell line (HTETOP) derivatives and Top2α expression constructs. K.I.P, J.R. and J.C.B.M.Z.

conceived the project, designed the experiments, analysed the data (with input from other authors) and, with the help of A.C.G.P., wrote the paper. CORRESPONDING AUTHORS Correspondence to

Konstantin I. Panov or Joost C. B. M. Zomerdijk. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY

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CITE THIS ARTICLE Ray, S., Panova, T., Miller, G. _et al._ Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation. _Nat

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