Transcriptome sequencing with the Genome Sequencer FLX system
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Transcriptome sequencing is a term that encompasses experiments including mRNA transcript-expression analysis (full-length mRNA, expressed sequence tags (ESTs) and ditags), novel gene
discovery, gene space identification in novel genomes, assembly of full-length genes, single-nucleotide polymorphism (SNP), insertion-deletion and splice-variant discovery, as well as
analyses of allele-specific expression and chromosomal rearrangement. The combination of long, accurate reads and high throughput makes 454 Sequencing™ analysis on the Genome Sequencer FLX
ideally suited to detailed transcriptome investigation.
This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the
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