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ABSTRACT Fast excitatory synaptic transmission through vertebrate autonomic ganglia is mediated by postsynaptic nicotinic acetylcholine receptors (nAChRs). We demonstrate a unique

postsynaptic receptor microheterogeneity on chick parasympathetic ciliary ganglion neurons—under one presynaptic terminal, nAChRs and glycine receptors formed separate but proximal clusters.

Terminals were loaded with [3H]glycine via the glycine transporter-1 (GlyT-1), which localized to the cholinergic presynaptic terminal membrane; depolarization evoked [3H]glycine release

that was calcium independent and blocked by the GlyT-1 inhibitor sarcosine. Ganglionic synaptic transmission mediated by nAChRs was attenuated by glycine. Coexistence of separate clusters of

receptors with opposing functions under one terminal contradicts Dale's principle and provides a new mechanism for modulating synaptic activity _ in vivo_. Access through your

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BEING VIEWED BY OTHERS LOCAL MODULATION BY PRESYNAPTIC RECEPTORS CONTROLS NEURONAL COMMUNICATION AND BEHAVIOUR Article 28 February 2022 NEUROTROPHIC FACTORS AND TARGET-SPECIFIC RETROGRADE

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BETWEEN STRIATAL CHOLINERGIC INTERNEURONS SHAPES THEIR NETWORK ACTIVITY PATTERNS IN A DOPAMINE-DEPENDENT MANNER Article Open access 09 October 2020 REFERENCES * Zhang, Z. W. & Berg, D.K.

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ACKNOWLEDGEMENTS We acknowledge Heinrich Betz for providing glycine receptor and gephyrin antibodies and clones, Yimen Ge (Massachusetts General Hospital, Harvard Medical School) for

assistance with the laser-scanning confocal microscope and Kathleen Dunlap, Daniel Jay and Tim Turner for advice and comments on the manuscript. This work was supported by NIH grant 21725 to

M.H.J. AUTHOR INFORMATION Author notes * The first two authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of Neuroscience, Tufts University School of Medicine,

136 Harrison Ave., Boston, 02111, Massachusetts, USA Guoshan Tsen, Brian Williams, Yu-Dong Zhou & Michele H. Jacob * Blackstone-Millville Regional High School, 175 Lincoln St.,

Blackstone, 01504, Massachusetts, USA Pauline Allaire * Dept. of Psychiatry, Wayne State University Med. Ctr. , 540 E. Canfield St., Detroit, 48201, Michigan, USA Ognian Ikonomov *

Department of Infectious Disease, Tufts University Veterinary School, 200 Westboro Rd., Grafton, 01536, Massachusetts, USA Ivanela Kondova Authors * Guoshan Tsen View author publications You

can also search for this author inPubMed Google Scholar * Brian Williams View author publications You can also search for this author inPubMed Google Scholar * Pauline Allaire View author

publications You can also search for this author inPubMed Google Scholar * Yu-Dong Zhou View author publications You can also search for this author inPubMed Google Scholar * Ognian Ikonomov

View author publications You can also search for this author inPubMed Google Scholar * Ivanela Kondova View author publications You can also search for this author inPubMed Google Scholar *

Michele H. Jacob View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Michele H. Jacob. RIGHTS AND PERMISSIONS

Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tsen, G., Williams, B., Allaire, P. _et al._ Receptors with opposing functions are in postsynaptic microdomains under one

presynaptic terminal. _Nat Neurosci_ 3, 126–132 (2000). https://doi.org/10.1038/72066 Download citation * Received: 09 September 1999 * Accepted: 09 December 1999 * Issue Date: February 2000

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