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Access through your institution Buy or subscribe > the mechanism of protection is not sequence specific CRISPR loci consist of multiple repeat sequences that are separated by variable

spacer sequences, which are often homologous to the protospacer segments of phage genomes or plasmids. The precursor transcript from CRISPR loci is processed into small CRISPR RNAs (crRNAs),

which, together with Cas proteins, guide the recognition and cleavage of foreign nucleic acid. To determine whether phages possess genes to counteract this system, the authors examined the

type I-F CRISPR–Cas system of _Pseudomonas aeruginosa_ str. PA14 using a collection of lysogenized strains, each containing a different prophage genome. To test whether the prophages

expressed anti-CRISPR activity, the ability of three CRISPR-sensitive phages to form plaques on these strains was measured. In contrast to unlysogenized _P. aeruginosa_ str. PA14, several

lysogenized strains supported the production of robust plaques, indicating that the integrated prophage inactivated the CRISPR–Cas system, allowing the CRISPR-sensitive phage to replicate.

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calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support ORIGINAL RESEARCH PAPER * Bondy-Denomy, J.

et al. Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. _Nature_ 16 Dec 2012 (doi:10.1038/nature11723) Article  PubMed  PubMed Central  Google Scholar  Download

references Authors * Christina Tobin Kåhrström View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS

ARTICLE CITE THIS ARTICLE Kåhrström, C. Giving CRISPR the slip. _Nat Rev Microbiol_ 11, 72 (2013). https://doi.org/10.1038/nrmicro2958 Download citation * Published: 03 January 2013 * Issue

Date: February 2013 * DOI: https://doi.org/10.1038/nrmicro2958 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

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