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ABSTRACT Bacterial motility involves switching between the left and right supercoiled states of the flagellar filament. The polymorphism of this assembly of identical flagellin molecules has

presented a structural puzzle. Supercoiling has been attributed to coexistence of two conformational states of the 11 nearly axially aligned protofilament strands of subunits. The helical

parameters of straight filaments in the left (L) and right (R) lattice states have now been accurately determined by X-ray fiber diffraction. The 9 Å resolution electron density map of the

R-type filament, refined from the X-ray data, reveals the interlocked α-helical segments of the core portion, which constitute the inner and outer tubes. While the inner-tube domain

interactions remain invariant, the strand joints in the outer tube can switch between the L- and R-state by 2–3 Å axial shifts, which change the strand periodicity of ∼50 Å by 0.8 Å. This

bi-stable quaternary switching results in supercoiling. Based on the measured helical parameters of the L and R lattices and the switching model, the twist and curvature calculated for the

ten possible supercoils are in quantitative accord with observed supercoiled forms of flagellar filaments. Access through your institution Buy or subscribe This is a preview of subscription

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BACTERIAL FLAGELLAR BASAL BODY Article 30 April 2021 THE CRYO-EM STRUCTURE OF THE BACTERIAL FLAGELLUM CAP COMPLEX SUGGESTS A MOLECULAR MECHANISM FOR FILAMENT ELONGATION Article Open access

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Scholar  Download references AUTHOR INFORMATION Author notes * Yuko Mimori-Kiyosue Present address: ERATO, Cell Axis Project, 17 Minamimachi, Nakadoji, Shimogyo, Kyoto, 600, Japan AUTHORS

AND AFFILIATIONS * International Institute for Advanced Research, Matsushita Electric Industrial Co., Ltd., 3-4 Hikaridai, Seika, 619-02, Japan llchiro Yamashita, Kazuya Hasegawa, Hirofumi

Suzuki, Ferenc Vonderviszt, Yuko Mimori-Kiyosue & Keiichi Namba * Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, 565, Japan Kazuya Hasegawa * Department of

Physics, University of Veszprém, Vészprem, Egyetem u.10., H-8201, Hungary Ferenc Vonderviszt Authors * llchiro Yamashita View author publications You can also search for this author inPubMed

 Google Scholar * Kazuya Hasegawa View author publications You can also search for this author inPubMed Google Scholar * Hirofumi Suzuki View author publications You can also search for this

author inPubMed Google Scholar * Ferenc Vonderviszt View author publications You can also search for this author inPubMed Google Scholar * Yuko Mimori-Kiyosue View author publications You

can also search for this author inPubMed Google Scholar * Keiichi Namba View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints

and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yamashita, l., Hasegawa, K., Suzuki, H. _et al._ Structure and switching of bacterial flagellar filaments studied by X-ray fiber

diffraction. _Nat Struct Mol Biol_ 5, 125–132 (1998). https://doi.org/10.1038/nsb0298-125 Download citation * Received: 29 July 1997 * Accepted: 29 December 1997 * Published: 01 February

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