Stimulation of chondrocyte dna synthesis by juvenile chronic arthritis synovial fluid
- Select a language for the TTS:
- UK English Female
- UK English Male
- US English Female
- US English Male
- Australian Female
- Australian Male
- Language selected: (auto detect) - EN
Play all audios:
ABSTRACT Synovial fluid from patients with juvenile chronic arthritis (JCA) was found to stimulate the DNA synthesis of rat epiphyseal chondrocytes in serum-free primary cultures in vitro.
The effect was dose-dependent and more pronounced than that observed with newborn calf serum. The cloning efficiency and colony size of chondrocytes in soft agar colony cultures were also
stimulated. JCA synovial fluid also contained interleukin-1 (IL-1) activity when tested in a murine thymocyte proliferation assay. AcA 54 gel filtration chromatography showed similar elution
pattern of the chondrocyte stimulating activity and the IL-1 activity, indicating that IL-1 might act as a chondrocyte growth factor in vitro. This assumption was further supported by
experiments demonstrating a dose—related stimulation of chondrocyte DNA synthesis by natural guinea pig macrophage—derived IL—1 and recombinant human IL—1. As it has been shown that the
articular cavity is vascularly connected with nearby epiphyseal plates, our results might give one explanation for the stimulation of bone growth often seen in the affected limb in children
with monoarthritis. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Pediatric Endocrinology Unit, Karolinska Hospital, D Söder & K Madsen (Introd. by E.M. Ritzén) * Department
of Histology, Karolinska Institute, Stockholm, Sweden D Söder & K Madsen (Introd. by E.M. Ritzén) Authors * D Söder View author publications You can also search for this author inPubMed
Google Scholar * K Madsen (Introd. by E.M. Ritzén) View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT
THIS ARTICLE CITE THIS ARTICLE Söder, D., Madsen, K. STIMULATION OF CHONDROCYTE DNA SYNTHESIS BY JUVENILE CHRONIC ARTHRITIS SYNOVIAL FLUID. _Pediatr Res_ 23, 112 (1988).
https://doi.org/10.1203/00006450-198801000-00066 Download citation * Issue Date: 01 January 1988 * DOI: https://doi.org/10.1203/00006450-198801000-00066 SHARE THIS ARTICLE Anyone you share
the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer
Nature SharedIt content-sharing initiative