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ABSTRACT Plant roots release a range of enzymes capable of degrading chemical compounds in their immediate vicinity1,2. We present a system of phytoremediation _ex planta_ based on the

overexpression of one such enzyme, a secretory laccase. Laccases catalyze the oxidation of a broad range of phenolic compounds3, including polychlorinated phenols such as

2,4,6-trichlorophenol (TCP), that are among the most hazardous and recalcitrant pollutants in the environment4. We isolated a secretory laccase cDNA of _LAC1_, which is specifically

expressed in the roots of _Gossypium arboreum_ (cotton). Transgenic _Arabidopsis thaliana_ plants overexpressing _LAC1_ exhibited enhanced resistance to several phenolic allelochemicals and

TCP. The secretory laccase activity in these plants was responsible for the conversion of sinapic acid into a mono-lactone type dimer and for the transformation of TCP. Access through your

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references ACKNOWLEDGEMENTS We thank J. Chen, W.L. Hu and Y.J. Lai for their help in HPLC-MS and GC-MS analysis. This research was supported by National Natural Sciences Foundation of China

(grants 30030020 and 39925005) and by the Chinese Academy of Sciences (grant KSCX2-SW-313). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * National Key Laboratory of Plant Molecular Genetics,

Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, P.R. China Guo-Dong Wang, Qian-Jin

Li, Bin Luo & Xiao-Ya Chen Authors * Guo-Dong Wang View author publications You can also search for this author inPubMed Google Scholar * Qian-Jin Li View author publications You can

also search for this author inPubMed Google Scholar * Bin Luo View author publications You can also search for this author inPubMed Google Scholar * Xiao-Ya Chen View author publications You

can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Xiao-Ya Chen. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing

financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG. 1 GUS staining of the 4-day-old Arabidopsis seedlings expressing _35S::GUS_ or _pLAC1::GUS_. (PDF 140 kb) SUPPLEMENTARY FIG.

2 Resistance of Arabidopsis plants of different transgenic LAC lines to TCP, 3 weeks after the second spraying. See also Figure 3. (PDF 182 kb) SUPPLEMENTARY FIG. 3 HPLC-MS analysis of

soluble phenolics of the 2-week-old WT and LAC 4-2 seedlings of Arabidopsis cultured in 1/2 MS medium (A) or in the medium containing 0.5 mM sinapic acid (B). (PDF 15 kb) SUPPLEMENTARY TABLE

1 Root elongation of WT and LAC 4-2 seedlings grown in the agar plate in the presence of TCP (PDF 18 kb) SUPPLEMENTARY TABLE 2 Root elongation of WT and LAC seedlings grown in the agar

plate in the presence of 20 μM TCP (PDF 16 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Wang, GD., Li, QJ., Luo, B. _et al._ _Ex planta_

phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase. _Nat Biotechnol_ 22, 893–897 (2004). https://doi.org/10.1038/nbt982 Download citation *

Received: 10 February 2004 * Accepted: 03 March 2004 * Published: 13 June 2004 * Issue Date: 01 July 2004 * DOI: https://doi.org/10.1038/nbt982 SHARE THIS ARTICLE Anyone you share the

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